Journal: Journal of Innate Immunity

Article Title: SR-A, MARCO and TLRs Differentially Recognise Selected Surface Proteins from Neisseria meningitidis : an Example of Fine Specificity in Microbial Ligand Recognition by Innate Immune Receptors

doi: 10.1159/000155227

Figure Lengend Snippet: Polyanionic ligands do not stimulate TLR-mediated cytokine induction. Bg-PMϕ from WT and MyD88–/– mice were stimulated with 1 µg/ml of each polyanionic ligand and the induction of IL-6 (a) and TNF-α (b) was measured. Cells were separately stimulated with E. coli LPS (100 ng/ml), which served as a positive control for cytokine induction.

Article Snippet: The ELISA kits for murine tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were from BD Biosciences Pharmingen (San Diego, Calif., USA).

Techniques: Positive Control

Journal: Journal of Innate Immunity

Article Title: SR-A, MARCO and TLRs Differentially Recognise Selected Surface Proteins from Neisseria meningitidis : an Example of Fine Specificity in Microbial Ligand Recognition by Innate Immune Receptors

doi: 10.1159/000155227

Figure Lengend Snippet: Selected N. meningitidis surface proteins stimulate TLR-mediated cytokine induction. Bg-PMϕ from WT and MyD88–/– mice were stimulated with 1 µg/ml of the purified recombinant N. meningitidis surface proteins and the induction of IL-6 (a) and TNF-α (b) was measured. Cells were separately stimulated with E. coli LPS (100 ng/ml), which served as a positive control for cytokine induction.

Article Snippet: The ELISA kits for murine tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were from BD Biosciences Pharmingen (San Diego, Calif., USA).

Techniques: Purification, Recombinant, Positive Control

Journal: Journal of Innate Immunity

Article Title: SR-A, MARCO and TLRs Differentially Recognise Selected Surface Proteins from Neisseria meningitidis : an Example of Fine Specificity in Microbial Ligand Recognition by Innate Immune Receptors

doi: 10.1159/000155227

Figure Lengend Snippet: Cytokine induction in SR-A–/–, MARCO–/– and double knock-out Mϕ. RPMϕ from WT, SR-A–/–, MARCO–/– and SR-A-MARCO–/– double knock-out animals were stimulated with 1 µg/ml of the N. meningitidis proteins that were showntobeTLR agonists in figure ​figure44 and the induction of IL-6 (a) and TNF-α (b) was measured. Each strain of Mϕ was also stimulated with E. coli LPS (100 ng/ml), which served as a positive control for cytokine induction.

Article Snippet: The ELISA kits for murine tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were from BD Biosciences Pharmingen (San Diego, Calif., USA).

Techniques: Knock-Out, Positive Control

Journal: Journal of Innate Immunity

Article Title: SR-A, MARCO and TLRs Differentially Recognise Selected Surface Proteins from Neisseria meningitidis : an Example of Fine Specificity in Microbial Ligand Recognition by Innate Immune Receptors

doi: 10.1159/000155227

Figure Lengend Snippet: Summary of receptor interaction with N. meningitidis proteins

Article Snippet: The ELISA kits for murine tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were from BD Biosciences Pharmingen (San Diego, Calif., USA).

Techniques:

Journal: Frontiers in Neurology

Article Title: Identification of Blood Biomarkers for Alzheimer's Disease Through Computational Prediction and Experimental Validation

doi: 10.3389/fneur.2018.01158

Figure Lengend Snippet: The list of 35 AD-related blood-secretory proteins.

Article Snippet: For ELISA experiments, commercial ELISA kits for proteins gelsolin (GSN), brain-derived neurotrophic factor (BDNF), metalloproteinase inhibitor 1 (TIMP1), pigment epithelium-derived factor (SERPINF1) and amyloid-like protein 2 (APLP2) were bought from Uscn Life Science Inc. (Wuhan, China).

Techniques:

Journal: Frontiers in Neurology

Article Title: Identification of Blood Biomarkers for Alzheimer's Disease Through Computational Prediction and Experimental Validation

doi: 10.3389/fneur.2018.01158

Figure Lengend Snippet: Validation of the 10 selected proteins between AD samples and health controls by ELISA experiment. (A) The concentration of protein GSN in serum samples of AD and control. (B) The concentration of protein BDNF in serum samples of AD and control. (C) The concentration of protein TIMP1 in serum samples of AD and control. (D) The concentration of protein SERPINF1 in serum samples of AD and control. (E) The concentration of protein ITPRIP in serum samples of AD and control. (F) The concentration of protein TMED10 in serum samples of AD and control. (G) The concentration of protein VLDLR in serum samples of AD and control. (H) The concentration of protein MAPK8 in serum samples of AD and control. (I) The concentration of protein APLP2 in serum samples of AD and control. (J) The concentration of protein MAPK1 in serum samples of AD and control. * p < 0.05 vs. controls; ** p < 0.05 vs. controls; *** p < 0.0005 vs. controls.

Article Snippet: For ELISA experiments, commercial ELISA kits for proteins gelsolin (GSN), brain-derived neurotrophic factor (BDNF), metalloproteinase inhibitor 1 (TIMP1), pigment epithelium-derived factor (SERPINF1) and amyloid-like protein 2 (APLP2) were bought from Uscn Life Science Inc. (Wuhan, China).

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Concentration Assay, Control

Journal: Frontiers in Neurology

Article Title: Identification of Blood Biomarkers for Alzheimer's Disease Through Computational Prediction and Experimental Validation

doi: 10.3389/fneur.2018.01158

Figure Lengend Snippet: The results of 10 proteins in computational prediction and experimental validation.

Article Snippet: For ELISA experiments, commercial ELISA kits for proteins gelsolin (GSN), brain-derived neurotrophic factor (BDNF), metalloproteinase inhibitor 1 (TIMP1), pigment epithelium-derived factor (SERPINF1) and amyloid-like protein 2 (APLP2) were bought from Uscn Life Science Inc. (Wuhan, China).

Techniques: Biomarker Discovery, Control

Journal: Frontiers in Neurology

Article Title: Identification of Blood Biomarkers for Alzheimer's Disease Through Computational Prediction and Experimental Validation

doi: 10.3389/fneur.2018.01158

Figure Lengend Snippet: Receiver operating characteristic curve analyses on the 5 proteins. The blue line represents protein APLP2, the red line is BDNF, the green line is GSN, the orange line is TIMP1 and the purple line is VLDLR.

Article Snippet: For ELISA experiments, commercial ELISA kits for proteins gelsolin (GSN), brain-derived neurotrophic factor (BDNF), metalloproteinase inhibitor 1 (TIMP1), pigment epithelium-derived factor (SERPINF1) and amyloid-like protein 2 (APLP2) were bought from Uscn Life Science Inc. (Wuhan, China).

Techniques:

Journal: Annals of Translational Medicine

Article Title: Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway

doi: 10.21037/atm-21-5898

Figure Lengend Snippet: NGR2 increased hypoxia in the colon mucosa with an increased ratio of serum VEGFA165/VEGFA121 and increased protein expression of Rap1GAP and TSP1. (A) The protein expression levels of VEGFR2, VEGFR2 (p1175), VEGFR2 (p1214), Rap1GAP, TSP1, and HIF-1α in the colon mucosa; (B,C) the serum contents of VEGFA165 and VEGFA121 and the ratio of VEGFA165/VEGFA121; (D,E) the concentration of intracellular pyruvate and lactic acid in the colonic mucosa. The data are means ± SEM of 3 independent experiments performed in triplicate. One-way ANOVA and Student’s t-test (**, P<0.001 vs. control).

Article Snippet: VEGFA121 (no: SEB851Ra) and VEGFA (no: SEA143Ra) ELISA kits were purchased from Cloud-Clone Co. Anti-VEGFR2 antibody (ab11939), anti-VEGFR2 (phospho Y1214) antibody (ab5475), and anti-Rap1GAP antibody (ab32373) were purchased from Abcam Co. (London, United Kingdom).

Techniques: Expressing, Concentration Assay, Control

Journal: Experimental Animals

Article Title: Emodin reactivated autophagy and alleviated inflammatory lung injury in mice with lethal endotoxemia

doi: 10.1538/expanim.19-0004

Figure Lengend Snippet: Emodin reduced the activity of pulmonary myeloperoxidase (MPO), the levels of pulmonary tumor necrosis factor alpha (TNF-α) and IL-6, nitric oxide (NO), lactic acid (LA) in lipopolysaccharide (LPS)-exposed mice. A. The activities of MPO in lung tissue were determined by ELISA. B. The levels of TNF-α in lung tissue were determined by ELISA. C. The levels of IL-6 in lung tissue were determined by ELISA. D. The levels of NO in lung tissue were determined by ELISA. E. The levels of LA in lung tissue were determined by ELISA. Emodin was administered in mice with intraperitoneal exposure to LPS and the lung samples were harvested 18 h post LPS exposure. Data are representative of four independent experiments and values were presented as mean ± SD (n=8).

Article Snippet: The ELISA kits for judgement of mouse tumor necrosis factor alpha (TNF-α) and IL-6 were generated by NeoBioscience Technology Co. (Shenzhen, China).

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay