Journal: Military Medical Research

Article Title: Oral administration of asparagine and 3-indolepropionic acid prolongs survival time of rats with traumatic colon injury

doi: 10.1186/s40779-022-00397-w

Figure Lengend Snippet: TCI induces acute dysfunctions of the rat intestine. a ELISA to detect indicator concentrations of rats in the naïve ( n = 15), sham ( n = 15) and TCI ( n = 13) groups. b IHC analysis to detect ZO-1 and occludin expression in the intestinal tissues at distances of 1 cm, 3 cm and 5 cm from the lesions. The histograms of IHC scores are on the right of IHC pictures. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns non-significant. TCI traumatic colon injury, IL-6 interleukin-6, TNF-α tumor necrosis factor-α, DAO diamine oxidase, ZO-1 zonula occludens 1, IHC immunohistochemical, ELISA enzyme-linked immunosorbent assay

Article Snippet: ELISA kits for rat tumor necrosis factor-α (TNF-α, Abbkine, Wuhan, China), interleukin-6 (IL-6, Abbkine), diamine oxidase (DAO, Enzyme-linked Biotechnology, Shanghai, China), D-lactate (Nanjing Jiancheng, Jiangsu, China) and endotoxin (Enzyme-linked Biotechnology) were used to detect the levels of the corresponding indicators according to the manufacturer’s protocols.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemical staining

Journal: Military Medical Research

Article Title: Oral administration of asparagine and 3-indolepropionic acid prolongs survival time of rats with traumatic colon injury

doi: 10.1186/s40779-022-00397-w

Figure Lengend Snippet: Coadministration of Asn and IPA ameliorates intestinal homeostasis dysregulation induced by TCI. a The flow diagram to depict the experimental processes of preparing rats with TCI that were administered with vehicle, Asn, IPA or Asn plus IPA ( n = 10 per group). b The survival curve to show the survival time of the rats as in a . c ELISA to detect indicator concentrations of rats with sham operation that were administered with vehicle ( n = 10) and TCI rats that were administered with vehicle ( n = 9), Asn ( n = 10), IPA ( n = 10) or Asn plus IPA ( n = 10). d The IHC analysis to detect ZO-1 and occludin expression in intestinal tissues of rats as in c . The histograms of IHC scores are below IHC pictures. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns non-significant. TCI traumatic colon injury, Asn asparagine, IPA 3-indolepropionic acid, IL-6 interleukin-6, TNF-α tumor necrosis factor-α, DAO diamine oxidase, ZO-1 zonula occludens 1, ELISA enzyme-linked immunosorbent assay, IHC immunohistochemical

Article Snippet: ELISA kits for rat tumor necrosis factor-α (TNF-α, Abbkine, Wuhan, China), interleukin-6 (IL-6, Abbkine), diamine oxidase (DAO, Enzyme-linked Biotechnology, Shanghai, China), D-lactate (Nanjing Jiancheng, Jiangsu, China) and endotoxin (Enzyme-linked Biotechnology) were used to detect the levels of the corresponding indicators according to the manufacturer’s protocols.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemical staining

Journal: Military Medical Research

Article Title: Oral administration of asparagine and 3-indolepropionic acid prolongs survival time of rats with traumatic colon injury

doi: 10.1186/s40779-022-00397-w

Figure Lengend Snippet: Asn plus IPA regimen facilitates intestinal homeostasis recovery by activating Akt signaling in the intestinal epithelium. a The pie charts to show the proportions of protein phosphorylation sites that are enriched in PI3K/Akt pathway among the sites with significantly altered phosphorylation levels (FC ≥ 1.3 or FC ≤ 0.7). b The antibody microarray images to show the landscape of detected protein expression and phosphorylation. Akt Thr308 phosphorylation levels are compared between vehicle, Asn and IPA groups. c Western blotting analysis to show protein expression and phosphorylation of intestinal epithelium of rats that received indicated treatment. d The flow diagram to depict the experimental processes of preparing rats with TCI that were administered with vehicle, MK-2206, Asn plus IPA or Asn plus IPA and MK-2206 ( n = 15 per group). e The survival curve to show the survival time of the rats as in d . f Western blotting analysis to show protein expression and phosphorylation of intestinal epithelium of rats with sham operation that were administered with vehicle ( n = 15) and TCI rats that were administered with vehicle ( n = 12), Asn plus IPA ( n = 15) or Asn plus IPA and MK-2206 ( n = 13). g – m The ELISA to detect indicator concentrations of rats as in f . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns non-significant. TCI traumatic colon injury, Asn asparagine, IPA 3-indolepropionic acid, IL-6 interleukin-6, TNF-α tumor necrosis factor-α, DAO diamine oxidase, mTOR mammalian target of rapamycin, CREB cAMP-response element binding protein, ELISA enzyme-linked immunosorbent assay, POD post-operative day

Article Snippet: ELISA kits for rat tumor necrosis factor-α (TNF-α, Abbkine, Wuhan, China), interleukin-6 (IL-6, Abbkine), diamine oxidase (DAO, Enzyme-linked Biotechnology, Shanghai, China), D-lactate (Nanjing Jiancheng, Jiangsu, China) and endotoxin (Enzyme-linked Biotechnology) were used to detect the levels of the corresponding indicators according to the manufacturer’s protocols.

Techniques: Phospho-proteomics, Microarray, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: BMC Complementary and Alternative Medicine

Article Title: Effect of Ampelopsis Radix on wound healing in scalded rats

doi: 10.1186/s12906-015-0751-z

Figure Lengend Snippet: VEGF levels in cutaneous scald injury. Abbreviations: CON, control; SSD, silver sulfadiazine; AJE, Ampelopsis japonica tuberous root ethanol extract; VEGF, vascular endothelial growth factor. Values expressed as mean ± standard error of the mean ( n = 5–8). *** P < 0.001 vs . control. # P < 0.05, ### P < 0.001 vs . Vaseline

Article Snippet: Rat tumor necrosis factor alpha (TNF-α) ELISA kits and rat vascular endothelial growth factor (VEGF) ELISA kits were purchased from Koma Biotech Inc. (Seoul, Korea).

Techniques: Control